SmartPfuTMDNA polymeraseis a thermo-stable enzyme which isolated from PyrococcusFuriosus. The recombinant protein of this enzyme is expres-sedin E. coli. This protein is a 90kDa protein and has 5’ to 3’ polymerase , 5’ to 3 exonuclease activity and 3’to 5’ exonuclease activity.